Single-step purification of full-length human androgen receptor
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چکیده
منابع مشابه
Single-step purification of full-length human androgen receptor
The full-length human androgen receptor with an N-terminal biotin acceptor peptide tag was overexpressed in Spodoptera frugiperda cells in the presence of 1 microM dihydrotestosterone. Site-specific biotinylation of BAP was achieved in vivo by co-expression of E. coli biotin holoenzyme synthetase. The androgen receptor was purified by single-step affinity chromatography using Streptavidin Mutei...
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ژورنال
عنوان ژورنال: Nuclear Receptor Signaling
سال: 2005
ISSN: 1550-7629,1550-7629
DOI: 10.1621/nrs.03001